Search results for "cell-free miRNAs"
showing 3 items of 3 documents
Detection of circulating miRNAs: comparative analysis of extracellular vesicle-incorporated miRNAs and cell-free miRNAs in whole plasma of prostate c…
2017
Abstract Background Circulating cell-free miRNAs have emerged as promising minimally-invasive biomarkers for early detection, prognosis and monitoring of cancer. They can exist in the bloodstream incorporated into extracellular vesicles (EVs) and ribonucleoprotein complexes. However, it is still debated if EVs contain biologically meaningful amounts of miRNAs and may provide a better source of miRNA biomarkers than whole plasma. The aim of this study was to systematically compare the diagnostic potential of prostate cancer-associated miRNAs in whole plasma and in plasma EVs. Methods RNA was isolated from whole plasma and plasma EV samples from a well characterised cohort of 50 patient with …
Diagnostic, prognostic and predictive value of cell-free miRNAs in prostate cancer: a systematic review
2016
Prostate cancer, the second most frequently diagnosed cancer in males worldwide, is estimated to be diagnosed in 1.1 million men per year. Introduction of PSA testing substantially improved early detection of prostate cancer, however it also led to overdiagnosis and subsequent overtreatment of patients with an indolent disease. Treatment outcome and management of prostate cancer could be improved by the development of non-invasive biomarker assays that aid in increasing the sensitivity and specificity of prostate cancer screening, help to distinguish aggressive from indolent disease and guide therapeutic decisions. Prostate cancer cells release miRNAs into the bloodstream, where they exist …
Plazmas miRNS kā multiplās sklerozes biomarķieri
2018
Vairākos pētījumos ir atklātas miRNS, tajā skaitā cirkulējošas miRNS, kuru daudzums ir izmainīts multiplā sklerozē. miRNS kā jaunu biomarķieru izpēte šai slimībai varētu palīdzēt diagnosticēt un prognozēt tās attīstību agrīnā stadijā. Šajā pētījumā ir prezentēti mūsu dati par dažām miRNS, kas tikadetektētas MS pacientu asins plazmā, kā izmainītas. Mēs salīdzinājām miRNS ekspresijas līmeņus MS un tās apakštipos ar veselo kontroli, lai atrastu potenciālās miRNS, kas var tikt izmantotas, kā MS biomarķieri. Galvenais mērķis bija validēt sekvenēšanas rezultātā atlasītās miRNS, kuru daudzums bija izmainīts MS pacientu asins plazmā neatkarīgā pacientu kopā. miRNS 484, kuras daudzums bija visvairāk…